Function: Trait Research
State/Province: North Carolina [NC]
Country: United States [US]
Position Title: Research Associate – Biochemist
Job ID: 3040
*Provide technical assistance to scientists within the BioAnalytics team in the area of biochemistry and/or small molecule analytics
*Assisting BioAnalytics team members in the design and implementation of experimental plans.
*Execute experimental plans with timeliness and high quality
*Development, optimization and validation of biochemical or biological assays in order to detect and quantify specific proteins or small molecules in plant and microbial tissues/extracts
*Collect and analyze plant tissue extracts for the activity and/or quantity of particular enzymes/proteins and/or small molecules of interest
*Recombinant protein expression and purification
*Routine maintainence of BioAnalytics equipment including flushing of FPLCs and calibration of spectrophotometers
*Data analysis and communication of results to supervisor
Knowledge, Skills & Experience: Critical Knoeledge:
*Minimum of a Bachelor's degree in Biochemistry, Biotechnology or a related field.
*Background in general biochemistry or analytical/wet bench chemistry; experience with proteins is essential
*This role requires an understanding of the principles of protein purification, enzyme kinetics, principles of assay development and validation, and considerations when working with proteins and biological samples. An understanding of principles of chromatography (preparative and analytical) is also desirable to the role.
*An understanding of basic statistics for experiment design and data analysis is desirable.
*Critical skills/experience includes:-
*Recombinant protein expression and purification-
*HPLC and FPLC experience-
*Biochemical or molecular assay development-
*Preparation of extracts from biological matrices for subsequent analysis of proteins or small molecules-
*Experience in a variety of biochemical techniques to include, but not limited to, SDS-PAGE, Western blotting, UV/Vis spectroscopy, enzyme assays and analysis of enzyme kinetics
*Proficiency with Microsoft Word and Excel
*Beneficial to the role:-
*Experience working with unstable proteins -
*Experience with plant systems (whole plants and/or plant cell systems)-
*Familiarity with Kaleidagraph, GraphPad, or other graphing/data analysis software
Critical Technical, Professional & Personal capabilities:
*Ability to work in a team environment.
*Ability to learn and work quickly and efficiently
*Organization - ability to keep track of tasks and manage own workload to meet timelines
Critical Success Factors & Key Challenges: *Ability to function independently
*Willingness and comfort to pro-actively consult with scientists and senior scientists within the group
*Ability to balance multiple tasks is essential.
*All applicants must be eligible to work in the US.
State or Province